|
KMID : 0903520070500010077
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 1 p.77 ~ p.81
|
|
|
Factors Affecting Pellet Formation of Phosphate-solubilizing Fungus, Aspergillus sp. PS-104 in Submerged Culture
|
|
Kang Sun-Chul
Shin Seung-Yong
|
|
|
Abstract
|
|
|
|
In order to minimize the mycelial pellet size of a high phosphate-solubilizing fungus, Aspergillus sp. PS-104 in liquid media, one of the critical obstacles during the submerged culture of filamentous fungi, an investigation was focused on the culture conditions (media and inoculum size) and additives (different soils, surfactants and polyethylene glycol 200). When the fungus was cultured in PDB, SDB and YPD media, their pellet sizes decreased in the order of SDB = YPD > PDB. At the higher concentrations of initial inoculum ranging from 1 × 103 to 1 × 107 conidia/ml, the smaller size of pellet was formed in the PDB medium. In addition, the pellet size was effectively reduced by 1/ 6~1/4 by the addition of 0.1% soil containing zeolite, diatomite, loess, kaoline and talc, excluding bentonite. The addition of 0.1% Tween 80, Triton X-100 and PEG 200 also decreased the pellet size, but SDS completely inhibited the fungal growth.
|
|
|
KEYWORD
|
|
|
pellet formation, phosphate-solubilizing fungus, Aspergillus sp., soil additives, PEG
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|